St Andrews Research Repository

St Andrews University Home
View Item 
  •   St Andrews Research Repository
  • Biology (School of)
  • Biology
  • Biology Theses
  • View Item
  •   St Andrews Research Repository
  • Biology (School of)
  • Biology
  • Biology Theses
  • View Item
  •   St Andrews Research Repository
  • Biology (School of)
  • Biology
  • Biology Theses
  • View Item
  • Login
JavaScript is disabled for your browser. Some features of this site may not work without it.

Production and characterisation of monoclonal and polyclonal antibodies against the transcription factor NF-kB and its inhibitor I-kB

Thumbnail
View/Open
ElizabethAnnWatsonMPhilThesis.pdf (21.58Mb)
Date
1995
Author
Watson, Elizabeth Ann.
Supervisor
Hay, R. T.
Metadata
Show full item record
Altmetrics Handle Statistics
Abstract
The activation of the transcription factor NF-kB and its inhibition by I-kB has been the subject of much research. However, there are many questions which remain unanswered regarding the functional properties of, and relationship between, the factor and the inhibitor. In this study, antibodies directed against the protein components of NF-kB and I-kB have been produced and used as tools to determine the subcellular localisation of both proteins in different cell lines both before and after activation with various agents. In particular the production and characterisation of monoclonal antibody 3B6 will be described. This antibody was raised against the p50 subunit of NF-kB and recognises an epitope contained within the nuclear localisation signal of the protein. Polyclonal antisera raised against p50 and I-kB were used in Western blots and immunofluorescence experiments and show changes in the localisation of NF-kB subunits and I-kB following activation of cells for various times.
Type
Thesis, MPhil Master of Philosophy
Collections
  • Biology Theses
URI
http://hdl.handle.net/10023/13885

Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

Advanced Search

Browse

All of RepositoryCommunities & CollectionsBy Issue DateNamesTitlesSubjectsClassificationTypeFunderThis CollectionBy Issue DateNamesTitlesSubjectsClassificationTypeFunder

My Account

Login

Open Access

To find out how you can benefit from open access to research, see our library web pages and Open Access blog. For open access help contact: openaccess@st-andrews.ac.uk.

Accessibility

Read our Accessibility statement.

How to submit research papers

The full text of research papers can be submitted to the repository via Pure, the University's research information system. For help see our guide: How to deposit in Pure.

Electronic thesis deposit

Help with deposit.

Repository help

For repository help contact: Digital-Repository@st-andrews.ac.uk.

Give Feedback

Cookie policy

This site may use cookies. Please see Terms and Conditions.

Usage statistics

COUNTER-compliant statistics on downloads from the repository are available from the IRUS-UK Service. Contact us for information.

© University of St Andrews Library

University of St Andrews is a charity registered in Scotland, No SC013532.

  • Facebook
  • Twitter