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dc.contributor.advisorPenedo Esteiro, Juan Carlos
dc.contributor.authorPérez González, Daniel Cibrán
dc.coverage.spatialxx, 194en_US
dc.description.abstractNucleic acids and proteins, some of the building blocks of life, are not static structures but highly dynamic entities that need to interact with one another to meet cellular demands. The work presented in this thesis focuses on the application of highly sensitive fluorescence methods, both at ensemble and single-molecule level, to determine the dynamics and structure of specific biomolecular interactions with nanometer resolution and in temporal scales from nanoseconds to minutes, which includes most biologically relevant processes. The main aims of my PhD can be classified in three areas: i) exploring new fluorescent sensors with increased specificity for certain nucleic acid structures; ii) understanding how some of these nucleic acids sense the presence of small molecules in the cellular environment and trigger gene regulation by altering their structure; and iii) understanding how certain molecular machines, such as helicase proteins, are able to unwind the DNA double helix by using chemical energy in the form of ATP hydrolysis.en_US
dc.publisherUniversity of St Andrews
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.subjectRNA foldingen_US
dc.subjectTIRF microscopyen_US
dc.subjectFluorescence resonance energy transfer (FRET)en_US
dc.subjectHelicase unwindingen_US
dc.subject.lcshNucleic acids--Analysis.en
dc.subject.lcshDNA-protein interactions.en
dc.subject.lcshRNA-protein interactions.en
dc.titleSingle-molecule studies of nucleic acid folding and nucleic acid-protein interactionsen_US
dc.contributor.sponsorEngineering and Physical Sciences Research Council (EPSRC)en_US
dc.contributor.sponsorUniversity of St Andrewsen_US
dc.type.qualificationnamePhD Doctor of Philosophyen_US
dc.publisher.institutionThe University of St Andrewsen_US
dc.rights.embargoreasonThesis restricted in accordance with University regulations. Electronic copy restricted until 29th August 2019en

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