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dc.contributor.authorZech, Jurgen
dc.contributor.authorGodfrey, E.L.
dc.contributor.authorMasai, H.
dc.contributor.authorHartsuiker, E.
dc.contributor.authorDalgaard, J.Z.
dc.date.accessioned2015-10-09T16:10:01Z
dc.date.available2015-10-09T16:10:01Z
dc.date.issued2015-07-22
dc.identifier.citationZech , J , Godfrey , E L , Masai , H , Hartsuiker , E & Dalgaard , J Z 2015 , ' The DNA-binding domain of S. Pombe Mrc1 (Claspin) acts to enhance stalling at replication barriers ' , PLoS One , vol. 10 , no. 7 , e0132595 . https://doi.org/10.1371/journal.pone.0132595en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 222611717
dc.identifier.otherPURE UUID: 7db208d0-7d69-4260-b3ae-7a44fa3b7705
dc.identifier.otherScopus: 84941591742
dc.identifier.otherWOS: 000358597100040
dc.identifier.urihttp://hdl.handle.net/10023/7639
dc.descriptionFunding: Marie Curie Cancer Care (J.Z. and J.Z.D.), Biotechnology and Biological Sciences Research Council (J.Z.D.) and Cancer Research UK (E.H.) supported this work.en
dc.description.abstractDuring S-phase replication forks can stall at specific genetic loci. At some loci, the stalling events depend on the replisome components Schizosaccharomyces pombe Swi1 (Saccharomyces cerevisiae Tof1) and Swi3 (S. cerevisiae Csm3) as well as factors that bind DNA in a site-specific manner. Using a new genetic screen we identified Mrc1 (S. cerevisiae Mrc1/ metazoan Claspin) as a replisome component involved in replication stalling. Mrc1 is known to form a sub-complex with Swi1 and Swi3 within the replisome and is required for the intra- S phase checkpoint activation. This discovery is surprising as several studies show that S. cerevisiae Mrc1 is not required for replication barrier activity. In contrast, we show that deletion of S. pombe mrc1 leads to an approximately three-fold reduction in barrier activity at several barriers and that Mrc1's role in replication fork stalling is independent of its role in checkpoint activation. Instead, S. pombe Mrc1 mediated fork stalling requires the presence of a functional copy of its phylogenetically conserved DNA binding domain. Interestingly, this domain is on the sequence level absent from S. cerevisiae Mrc1. Our study indicates that direct interactions between the eukaryotic replisome and the DNA are important for site-specific replication stalling.
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.rights© 2015 Zech et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleThe DNA-binding domain of S. Pombe Mrc1 (Claspin) acts to enhance stalling at replication barriersen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0132595
dc.description.statusPeer revieweden
dc.identifier.urlhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132595#sec022en


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