Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorSwanson, Maud M.
dc.contributor.authorReavy, Brian
dc.contributor.authorMakarova, Kira S.
dc.contributor.authorCock, Peter J.
dc.contributor.authorHopkins, David W.
dc.contributor.authorTorrance, Lesley
dc.contributor.authorKoonin, Eugene V.
dc.contributor.authorTaliansky, Michael
dc.date.accessioned2013-11-04T12:01:00Z
dc.date.available2013-11-04T12:01:00Z
dc.date.issued2012-07-17
dc.identifier.citationSwanson , M M , Reavy , B , Makarova , K S , Cock , P J , Hopkins , D W , Torrance , L , Koonin , E V & Taliansky , M 2012 , ' Novel bacteriophages containing a genome of another bacteriophage within their genomes ' , PLoS One , vol. 7 , no. 7 , e40683 . https://doi.org/10.1371/journal.pone.0040683en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 28341545
dc.identifier.otherPURE UUID: 3b32fb9c-1fbe-42f3-a17a-97b5a5205856
dc.identifier.otherWOS: 000306507000025
dc.identifier.otherScopus: 84863992182
dc.identifier.urihttps://hdl.handle.net/10023/4144
dc.descriptionThe work was funded by the Scottish Government’s Rural and Environment Science and Analytical Services (RESAS) Divisionen
dc.description.abstractA novel bacteriophage infecting Staphylococus pasteuri was isolated during a screen for phages in Antarctic soils. The phage named SpaA1 is morphologically similar to phages of the family Siphoviridae. The 42,784 bp genome of SpaA1 is a linear, double-stranded DNA molecule with 39 protruding cohesive ends. The SpaA1 genome encompasses 63 predicted protein-coding genes which cluster within three regions of the genome, each of apparently different origin, in a mosaic pattern. In two of these regions, the gene sets resemble those in prophages of Bacillus thuringiensis kurstaki str. T03a001 (genes involved in DNA replication/transcription, cell entry and exit) and B. cereus AH676 (additional regulatory and recombination genes), respectively. The third region represents an almost complete genome (except for the short terminal segments) of a distinct bacteriophage, MZTP02. Nearly the same gene module was identified in prophages of B. thuringiensis serovar monterrey BGSC 4AJ1 and B. cereus Rock4-2. These findings suggest that MZTP02 can be shuttled between genomes of other bacteriophages and prophages, leading to the formation of chimeric genomes. The presence of a complete phage genome in the genome of other phages apparently has not been described previously and might represent a 'fast track' route of virus evolution and horizontal gene transfer. Another phage (BceA1) nearly identical in sequence to SpaA1, and also including the almost complete MZTP02 genome within its own genome, was isolated from a bacterium of the B. cereus/B. thuringiensis group. Remarkably, both SpaA1 and BceA1 phages can infect B. cereus and B. thuringiensis, but only one of them, SpaA1, can infect S. pasteuri. This finding is best compatible with a scenario in which MZTP02 was originally contained in BceA1 infecting Bacillus spp, the common hosts for these two phages, followed by emergence of SpaA1 infecting S. pasteuri.
dc.format.extent12
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.rights© 2012 Swanson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectNovel bacteriophageen
dc.subjectSpaA1 genomeen
dc.subjectDNA replicationen
dc.subjectMZTP02en
dc.subjectChimeric genomesen
dc.subjectVirus evolutionen
dc.subjectHorizontal gene transferen
dc.subjectQ Scienceen
dc.subject.lccQen
dc.titleNovel bacteriophages containing a genome of another bacteriophage within their genomesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0040683
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record