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Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata

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dc.contributor.authorBarr, Zoe
dc.contributor.authorTilsner, Jens
dc.contributor.editorHussey, Patrick J.
dc.contributor.editorWang, Pengwei
dc.date.accessioned2025-02-12T00:45:49Z
dc.date.available2025-02-12T00:45:49Z
dc.date.issued2023-02-12
dc.identifier283396639
dc.identifier8ad4baa1-15d0-4cac-845f-151fa968e6ce
dc.identifier85147892786
dc.identifier.citationBarr , Z & Tilsner , J 2023 , Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata . in P J Hussey & P Wang (eds) , The plant cytoskeleton : methods and protocols . Methods in molecular biology , vol. 2604 , Humana Press , New York, NY , pp. 193-202 . https://doi.org/10.1007/978-1-0716-2867-6_14en
dc.identifier.isbn9781071628669
dc.identifier.isbn9781071628690
dc.identifier.isbn9781071628676
dc.identifier.issn1064-3745
dc.identifier.otherRIS: urn:2B950E6D4DBD91EF48A6973E78E614EA
dc.identifier.otherRIS: Barr2023
dc.identifier.otherORCID: /0000-0003-3873-0650/work/129147775
dc.identifier.urihttps://hdl.handle.net/10023/31368
dc.descriptionFunding: Z.B. is funded by the UK Biotechnology and Biological Sciences Research Council EASTBIO Doctoral Training Partnership. The Tilsner lab receives funding from the Scottish Government Rural and Environment Science and Analytical Services (RESAS).en
dc.description.abstractThe actin cytoskeleton has close but so far incompletely understood connections to plasmodesmata, the cell junctions of plants. Plasmodesmata are essential for plant development and responses to biotic and abiotic stresses and facilitate the intercellular exchange of metabolites and hormones but also macromolecules such as proteins and RNAs. The molecular size exclusion limited of plasmodesmata is dynamically regulated, including by actin-associated proteins. Therefore, experimental analysis of plasmodesmal regulation can be relevant to plant cytoskeleton research. This chapter presents two simple imaging-based protocols for analyzing macromolecular cell-to-cell connectivity in leaves.
dc.format.extent10
dc.format.extent442834
dc.language.isoeng
dc.publisherHumana Press
dc.relation.ispartofThe plant cytoskeletonen
dc.relation.ispartofseriesMethods in molecular biologyen
dc.rightsCopyright © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1007/978-1-0716-2867-6_14.en
dc.subjectQH301 Biologyen
dc.subjectSB Plant cultureen
dc.subjectMCCen
dc.subject.lccQH301en
dc.subject.lccSBen
dc.titleCell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmataen
dc.typeBook itemen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Centre for Biophotonicsen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doi10.1007/978-1-0716-2867-6_14
dc.date.embargoedUntil2025-02-12
dc.identifier.urlhttps://doi.org/10.1007/978-1-0716-2867-6en
dc.identifier.urlhttps://discover.libraryhub.jisc.ac.uk/search?isn=9781071628669&rn=1en


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