Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata
Date
12/02/2023Metadata
Show full item recordAltmetrics Handle Statistics
Altmetrics DOI Statistics
Abstract
The actin cytoskeleton has close but so far incompletely understood connections to plasmodesmata, the cell junctions of plants. Plasmodesmata are essential for plant development and responses to biotic and abiotic stresses and facilitate the intercellular exchange of metabolites and hormones but also macromolecules such as proteins and RNAs. The molecular size exclusion limited of plasmodesmata is dynamically regulated, including by actin-associated proteins. Therefore, experimental analysis of plasmodesmal regulation can be relevant to plant cytoskeleton research. This chapter presents two simple imaging-based protocols for analyzing macromolecular cell-to-cell connectivity in leaves.
Citation
Barr , Z & Tilsner , J 2023 , Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata . in P J Hussey & P Wang (eds) , The plant cytoskeleton : methods and protocols . Methods in molecular biology , vol. 2604 , Humana Press , New York, NY , pp. 193-202 . https://doi.org/10.1007/978-1-0716-2867-6_14
Publication
The plant cytoskeleton
ISSN
1064-3745Type
Book item
Rights
Copyright © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1007/978-1-0716-2867-6_14.
Description
Funding: Z.B. is funded by the UK Biotechnology and Biological Sciences Research Council EASTBIO Doctoral Training Partnership. The Tilsner lab receives funding from the Scottish Government Rural and Environment Science and Analytical Services (RESAS).Collections
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.