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dc.contributor.authorFox, Stephen
dc.contributor.authorGoswami, Cosmika
dc.contributor.authorHolden, Matthew
dc.contributor.authorConnolly, James P. R.
dc.contributor.authorMordue, James
dc.contributor.authorO’Boyle, Nicky
dc.contributor.authorRoe, Andrew
dc.contributor.authorConnor, Martin
dc.contributor.authorLeanord, Alistair
dc.contributor.authorEvans, Tom J.
dc.date.accessioned2020-03-16T15:30:02Z
dc.date.available2020-03-16T15:30:02Z
dc.date.issued2020-03-05
dc.identifier.citationFox , S , Goswami , C , Holden , M , Connolly , J P R , Mordue , J , O’Boyle , N , Roe , A , Connor , M , Leanord , A & Evans , T J 2020 , ' A highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineage ' , Scientific Reports , vol. 10 , 4135 . https://doi.org/10.1038/s41598-020-61026-xen
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 266866561
dc.identifier.otherPURE UUID: 42399abb-79ac-4cae-9b23-c8575b64757e
dc.identifier.otherRIS: urn:B90FF352961BC66CCEEBA295B46B8D2F
dc.identifier.otherRIS: Fox2020
dc.identifier.otherORCID: /0000-0002-4958-2166/work/70619060
dc.identifier.otherScopus: 85081330268
dc.identifier.otherWOS: 000563316900004
dc.identifier.urihttp://hdl.handle.net/10023/19662
dc.descriptionThe work was funded by the Scottish Executive via the Chief Scientists Office through the provision of a grant to establish the Scottish Healthcare Associated Infection Prevention Institute (SHAIPI). The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.en
dc.description.abstractBacterial type III secretion systems (T3SSs) play an important role in pathogenesis of Gram-negative infections. Enteropathogenic and enterohemorrhagic Escherichia coli contain a well-defined T3SS but in addition a second T3SS termed E. coli T3SS 2 (ETT2) has been described in a number of strains of E. coli. The majority of pathogenic E. coli contain elements of a genetic locus encoding ETT2, but which has undergone significant mutational attrition rendering it without predicted function. Only a very few strains have been reported to contain an intact ETT2 locus. To investigate the occurrence of the ETT2 locus in strains of human pathogenic E. coli, we carried out genomic sequencing of 162 isolates obtained from patient blood cultures in Scotland. We found that 22 of 26 sequence type (ST) 69 isolates from this collection contained an intact ETT2 together with an associated eip locus which encodes putative secreted ETT2 effectors as well as eilA, a gene encoding a putative transcriptional regulator of ETT2 associated genes. Using a reporter gene for eilA activation, we defined conditions under which this gene was differentially activated. Analysis of published E. coli genomes with worldwide representation showed that ST69 contained an intact ETT2 in these strains as well. The conservation of the genes encoding ETT2 in human pathogenic ST69 strains strongly suggests it has importance in infection, although its exact functional role remains obscure.
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsCopyright © 2020 the Author(s). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectBacterial geneticsen
dc.subjectGenetics researchen
dc.subjectQH426 Geneticsen
dc.subjectQR Microbiologyen
dc.subjectRB Pathologyen
dc.subjectDASen
dc.subject.lccQH426en
dc.subject.lccQRen
dc.subject.lccRBen
dc.titleA highly conserved complete accessory Escherichia coli type III secretion system 2 is widespread in bloodstream isolates of the ST69 lineageen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.identifier.doihttps://doi.org/10.1038/s41598-020-61026-x
dc.description.statusPeer revieweden


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