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dc.contributor.authorShah, Anokhi
dc.contributor.authorRoux, Amandine
dc.contributor.authorStarck, Matthieu
dc.contributor.authorMosely, Jackie A.
dc.contributor.authorStevens, Michael
dc.contributor.authorNorman, David G.
dc.contributor.authorHunter, Robert I.
dc.contributor.authorEl Mkami, Hassane
dc.contributor.authorSmith, Graham
dc.contributor.authorParker, David
dc.contributor.authorLovett, Janet E.
dc.date.accessioned2020-02-18T00:34:57Z
dc.date.available2020-02-18T00:34:57Z
dc.date.issued2019-02-18
dc.identifier.citationShah , A , Roux , A , Starck , M , Mosely , J A , Stevens , M , Norman , D G , Hunter , R I , El Mkami , H , Smith , G , Parker , D & Lovett , J E 2019 , ' A gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurements ' , Inorganic Chemistry , vol. Article ASAP . https://doi.org/10.1021/acs.inorgchem.8b02892en
dc.identifier.issn0020-1669
dc.identifier.otherPURE: 257572157
dc.identifier.otherPURE UUID: dcb76899-56c3-42a6-a2e7-06fe98a046b8
dc.identifier.otherORCID: /0000-0002-3561-450X/work/54516612
dc.identifier.otherScopus: 85062371195
dc.identifier.otherORCID: /0000-0001-6578-7412/work/59464751
dc.identifier.otherORCID: /0000-0002-0552-5784/work/60195391
dc.identifier.otherORCID: /0000-0002-0415-9540/work/60195457
dc.identifier.otherWOS: 000460600300014
dc.identifier.urihttps://hdl.handle.net/10023/19485
dc.descriptionFunding from ERC (266804), EPSRC (EP/R013705/1, EP/M508214/1), Carnegie Trust (RIG007510), Wellcome Trust for a Multi-User Equipment Grant (099149/Z/12/Z), and the Royal Society for a University Research Fellowship to JEL.en
dc.description.abstractThe design, synthesis, and application of a nine-coordinate gadolinium(III)-containing spin label, [Gd.sTPATCN]-SL, for use in nanometer-distance measurement experiments by EPR spectroscopy is presented. The spin label links to cysteines via a short thioether tether and has a narrow central transition indicative of small zero-field splitting (ZFS). A protein homodimer, TRIM25cc, was selectively labeled with [Gd.sTPATCN]-SL (70%) and a nitroxide (30%) under mild conditions and measured using the double electron electron resonance (DEER) technique with both commercial Q-band and home-built W-band spectrometers. The label shows great promise for increasing the sensitivity of DEER measurements through both its favorable relaxation parameters and the large DEER modulation depth at both Q- and W-band for the inter-Gd(III) DEER measurement which, at 9%, is the largest recorded under these conditions.
dc.language.isoeng
dc.relation.ispartofInorganic Chemistryen
dc.rightsCopyright © 2019 American Chemical Society. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at 10.1021/acs.inorgchem.8b02892en
dc.subjectQC Physicsen
dc.subjectQD Chemistryen
dc.subjectTK Electrical engineering. Electronics Nuclear engineeringen
dc.subjectDASen
dc.subject.lccQCen
dc.subject.lccQDen
dc.subject.lccTKen
dc.titleA gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurementsen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorThe Royal Societyen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1021/acs.inorgchem.8b02892
dc.description.statusPeer revieweden
dc.date.embargoedUntil2020-02-18
dc.identifier.grantnumberEP/R013705/1en
dc.identifier.grantnumber099149/Z/12/Zen
dc.identifier.grantnumberUF150698en


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