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A gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurements
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dc.contributor.author | Shah, Anokhi | |
dc.contributor.author | Roux, Amandine | |
dc.contributor.author | Starck, Matthieu | |
dc.contributor.author | Mosely, Jackie A. | |
dc.contributor.author | Stevens, Michael | |
dc.contributor.author | Norman, David G. | |
dc.contributor.author | Hunter, Robert I. | |
dc.contributor.author | El Mkami, Hassane | |
dc.contributor.author | Smith, Graham | |
dc.contributor.author | Parker, David | |
dc.contributor.author | Lovett, Janet E. | |
dc.date.accessioned | 2020-02-18T00:34:57Z | |
dc.date.available | 2020-02-18T00:34:57Z | |
dc.date.issued | 2019-02-18 | |
dc.identifier | 257572157 | |
dc.identifier | dcb76899-56c3-42a6-a2e7-06fe98a046b8 | |
dc.identifier | 85062371195 | |
dc.identifier | 000460600300014 | |
dc.identifier.citation | Shah , A , Roux , A , Starck , M , Mosely , J A , Stevens , M , Norman , D G , Hunter , R I , El Mkami , H , Smith , G , Parker , D & Lovett , J E 2019 , ' A gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurements ' , Inorganic Chemistry , vol. Article ASAP . https://doi.org/10.1021/acs.inorgchem.8b02892 | en |
dc.identifier.issn | 0020-1669 | |
dc.identifier.other | ORCID: /0000-0002-3561-450X/work/54516612 | |
dc.identifier.other | ORCID: /0000-0001-6578-7412/work/59464751 | |
dc.identifier.other | ORCID: /0000-0002-0552-5784/work/60195391 | |
dc.identifier.other | ORCID: /0000-0002-0415-9540/work/60195457 | |
dc.identifier.uri | https://hdl.handle.net/10023/19485 | |
dc.description | Funding from ERC (266804), EPSRC (EP/R013705/1, EP/M508214/1), Carnegie Trust (RIG007510), Wellcome Trust for a Multi-User Equipment Grant (099149/Z/12/Z), and the Royal Society for a University Research Fellowship to JEL. | en |
dc.description.abstract | The design, synthesis, and application of a nine-coordinate gadolinium(III)-containing spin label, [Gd.sTPATCN]-SL, for use in nanometer-distance measurement experiments by EPR spectroscopy is presented. The spin label links to cysteines via a short thioether tether and has a narrow central transition indicative of small zero-field splitting (ZFS). A protein homodimer, TRIM25cc, was selectively labeled with [Gd.sTPATCN]-SL (70%) and a nitroxide (30%) under mild conditions and measured using the double electron electron resonance (DEER) technique with both commercial Q-band and home-built W-band spectrometers. The label shows great promise for increasing the sensitivity of DEER measurements through both its favorable relaxation parameters and the large DEER modulation depth at both Q- and W-band for the inter-Gd(III) DEER measurement which, at 9%, is the largest recorded under these conditions. | |
dc.format.extent | 4798464 | |
dc.language.iso | eng | |
dc.relation.ispartof | Inorganic Chemistry | en |
dc.subject | QC Physics | en |
dc.subject | QD Chemistry | en |
dc.subject | TK Electrical engineering. Electronics Nuclear engineering | en |
dc.subject | DAS | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | TK | en |
dc.title | A gadolinium spin label with both a narrow central transition and short tether for use in double electron electron resonance distance measurements | en |
dc.type | Journal article | en |
dc.contributor.sponsor | EPSRC | en |
dc.contributor.sponsor | The Wellcome Trust | en |
dc.contributor.sponsor | The Royal Society | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1021/acs.inorgchem.8b02892 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2020-02-18 | |
dc.identifier.grantnumber | EP/R013705/1 | en |
dc.identifier.grantnumber | 099149/Z/12/Z | en |
dc.identifier.grantnumber | UF150698 | en |
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