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dc.contributor.advisorLovett, Janet Eleanor
dc.contributor.authorShah, Anokhi
dc.coverage.spatialxxvi, 195, [3] p.en_US
dc.description.abstractElectron paramagnetic resonance spectroscopy is a versatile tool for probing structural information about systems with unpaired electrons, in particular, biological systems with metal centres or chemically attached spin labels. This work uses a variety of electron paramagnetic resonance techniques to investigate the ability to measure distances between two spins (radical and metal), as well as characterise the local environment. Distance measurements are used to probe the mechanism of translocation across a membrane, where a change in distance is observed upon ATP cycling, indicating channel movement of the SecYEG:SecA complex. Furthermore, to expand the scope of distance measurements for more complex, cysteine-rich systems, spin labelling regimes are developed and optimised on the test protein myoglobin. Specifically, next generation maleimide spin labels are demonstrated to label and selectively cleave. Myoglobin is further exploited to successfully introduce the unnatural amino acid, dehydroalanine, for selective and orthogonal labelling. The development of the labelling and measurement strategy for the gadolinium-based spin label, [Gd.sTPATCN]-SL, is also shown, where the narrow central transition of the label allows a long phase memory time and increased DEER modulation depth, to give increased measurement sensitivity. In addition, gadolinium(III) distances are used to characterise the binding site of a peptide system for the application of magnetic resonance imaging. The optimisation of measuring inter-gadolinium(III) distances between 2-5 nm at both Q- and W-band is also demonstrated in the corresponding peptide ruler series. The additional benefit of the peptide to act as a metal ruler is further investigated using copper(II), where hyperfine spectroscopy is utilised to successfully confirm the nature of the binding site as all oxygen binding.en_US
dc.description.sponsorship"This work was supported by the Engineering and Physical Sciences Research Council [grant number EP/M508214/1]" -- Acknowledgementsen
dc.publisherUniversity of St Andrews
dc.relationExpanding the scope of EPR for structural studies of proteins and peptides (Thesis data) Shah, A., University of St Andrews. DOI:
dc.subjectElectron paramagnetic resonanceen_US
dc.subjectMass spectrometryen_US
dc.subjectSpin labellingen_US
dc.subjectNew labelsen_US
dc.subjectStructural biologyen_US
dc.subjectMembrane proteinen_US
dc.subject.lcshElectron paramagnetic resonanceen
dc.subject.lcshElectron paramagnetic resonance spectroscopyen
dc.subject.lcshMolecular biologyen
dc.subject.lcshMolecular structureen
dc.titleExpanding the scope of electron paramagnetic resonance spectroscopy for structural studies of proteins and peptidesen_US
dc.contributor.sponsorEngineering and Physical Sciences Research Council (EPSRC)en_US
dc.type.qualificationnamePhD Doctor of Philosophyen_US
dc.publisher.institutionThe University of St Andrewsen_US
dc.rights.embargoreasonThesis restricted in accordance with University regulations. Electronic copy restricted until 13th September 2024.en

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