Now showing items 1-10 of 51
A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus
(2012-06) - Journal article,
The removal of chemically damaged DNA bases such as 3-methyladenine (3-MeA) is an essential process in all living organisms and is catalyzed by the enzyme 3-MeA DNA glycosylase I. A key question is how the enzyme selectively ...
The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster
(2014-12) - Journal article,
Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed ...
The role of lipids in mechanosensation
(2015) - Journal article,
The ability of proteins to sense membrane tension is pervasive in biology. A higher-resolution structure of the Escherichia coli small-conductance mechanosensitive channel MscS identifies alkyl chains inside pockets formed ...
Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDOR
(2013-01) - Journal article,
Site-directed spin labeling and pulsed electron–electron double resonance (PELDOR or DEER) have previously been applied successfully to study the structure and dynamics of nucleic acids. Spin labeling nucleic acids at ...
An efficient method for the in vitro production of Azol(in)e-based cyclic peptides
(2014-12-15) - Journal article,
Heterocycle-containing cyclic peptides are promising scaffolds for the pharmaceutical industry but their chemical synthesis is very challenging. A new universal method has been devised to prepare these compounds by using ...
Derivatisable cyanobactin analogues : a semisynthetic approach
(2015-12-14) - Journal article,
Many natural cyclic peptides have potent and potentially useful biological activities. Their use as therapeutic starting points is often limited by the quantities available, the lack of known biological targets and the ...
Multiple nucleic acid cleavage modes in divergent type III CRISPR systems
(2016-02-29) - Journal article,
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of ...
The cyanobactin heterocyclase enzyme : A processive adenylase that operates with a defined order of reaction
(2013-12) - Journal article,
Structural analysis of leader peptide binding enables leader-free cyanobactin processing
(2015-08) - Journal article,
Regioselective modification of amino acids within the context of a peptide is common to a number of biosynthetic pathways and many such products have potential as therapeutics. The ATP dependent enzyme LynD heterocyclizes ...
High affinity RNA binding by a hyperthermophilic single-stranded DNA binding protein
(2017-03) - Journal article,
Single-stranded DNA binding proteins (SSBs), including replication protein A (RPA) in eukaryotes, play a central role in DNA replication, recombination and repair. SSBs utilise an oligomerise in solution to bring multiple ...