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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing

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dc.contributor.authorBossé, Janine T
dc.contributor.authorLi, Yanwen
dc.contributor.authorFernandez Crespo, Roberto
dc.contributor.authorLacouture, Sonia
dc.contributor.authorGottschalk, Marcelo
dc.contributor.authorSárközi, Rita
dc.contributor.authorFodor, László
dc.contributor.authorCasas Amoribieta, Maria
dc.contributor.authorAngen, Øystein
dc.contributor.authorNedbalcova, Katerina
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorMaskell, Duncan J
dc.contributor.authorTucker, Alexander W
dc.contributor.authorWren, Brendan W
dc.contributor.authorRycroft, Andrew N
dc.contributor.authorLangford, Paul R
dc.contributor.authorBRaDP1T Consortium
dc.date.accessioned2018-06-12T08:30:06Z
dc.date.available2018-06-12T08:30:06Z
dc.date.issued2018-07
dc.identifier253333898
dc.identifierf32264b2-4a43-44e8-8fd0-a7deba86a6dd
dc.identifier29885806
dc.identifier85047383347
dc.identifier000437041800013
dc.identifier.citationBossé , J T , Li , Y , Fernandez Crespo , R , Lacouture , S , Gottschalk , M , Sárközi , R , Fodor , L , Casas Amoribieta , M , Angen , Ø , Nedbalcova , K , Holden , M T G , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R & BRaDP1T Consortium 2018 , ' Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing ' , Veterinary Microbiology , vol. 220 , pp. 83-89 . https://doi.org/10.1016/j.vetmic.2018.05.011en
dc.identifier.issn0378-1135
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196426
dc.identifier.urihttps://hdl.handle.net/10023/13921
dc.descriptionThis work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1 and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MTGH was supported by the Wellcome Trust (grant number 098051). RS and LF were supported by the Hungarian Scientific Research Fund (OTKA 112826). Genome sequencing provided by MicrobesNG (www.microbesng.uk) was supported by the BBSRC (grant number BB/L024209/1).en
dc.description.abstractProblems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.
dc.format.extent7
dc.format.extent1388646
dc.language.isoeng
dc.relation.ispartofVeterinary Microbiologyen
dc.subjectDiagnosticen
dc.subjectmPCRen
dc.subjectCapsule typingen
dc.subjectA. pleuropneumoniaeen
dc.subjectSerovarsen
dc.subjectQR Microbiologyen
dc.subjectQH301 Biologyen
dc.subjectDASen
dc.subject.lccQRen
dc.subject.lccQH301en
dc.titleComparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typingen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Infection and Global Health Divisionen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. Infection Groupen
dc.identifier.doihttps://doi.org/10.1016/j.vetmic.2018.05.011
dc.description.statusPeer revieweden


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