Files in this item
Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing
Item metadata
dc.contributor.author | Bossé, Janine T | |
dc.contributor.author | Li, Yanwen | |
dc.contributor.author | Fernandez Crespo, Roberto | |
dc.contributor.author | Lacouture, Sonia | |
dc.contributor.author | Gottschalk, Marcelo | |
dc.contributor.author | Sárközi, Rita | |
dc.contributor.author | Fodor, László | |
dc.contributor.author | Casas Amoribieta, Maria | |
dc.contributor.author | Angen, Øystein | |
dc.contributor.author | Nedbalcova, Katerina | |
dc.contributor.author | Holden, Matthew T. G. | |
dc.contributor.author | Maskell, Duncan J | |
dc.contributor.author | Tucker, Alexander W | |
dc.contributor.author | Wren, Brendan W | |
dc.contributor.author | Rycroft, Andrew N | |
dc.contributor.author | Langford, Paul R | |
dc.contributor.author | BRaDP1T Consortium | |
dc.date.accessioned | 2018-06-12T08:30:06Z | |
dc.date.available | 2018-06-12T08:30:06Z | |
dc.date.issued | 2018-07 | |
dc.identifier | 253333898 | |
dc.identifier | f32264b2-4a43-44e8-8fd0-a7deba86a6dd | |
dc.identifier | 29885806 | |
dc.identifier | 85047383347 | |
dc.identifier | 000437041800013 | |
dc.identifier.citation | Bossé , J T , Li , Y , Fernandez Crespo , R , Lacouture , S , Gottschalk , M , Sárközi , R , Fodor , L , Casas Amoribieta , M , Angen , Ø , Nedbalcova , K , Holden , M T G , Maskell , D J , Tucker , A W , Wren , B W , Rycroft , A N , Langford , P R & BRaDP1T Consortium 2018 , ' Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing ' , Veterinary Microbiology , vol. 220 , pp. 83-89 . https://doi.org/10.1016/j.vetmic.2018.05.011 | en |
dc.identifier.issn | 0378-1135 | |
dc.identifier.other | ORCID: /0000-0002-4958-2166/work/60196426 | |
dc.identifier.uri | https://hdl.handle.net/10023/13921 | |
dc.description | This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1 and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MTGH was supported by the Wellcome Trust (grant number 098051). RS and LF were supported by the Hungarian Scientific Research Fund (OTKA 112826). Genome sequencing provided by MicrobesNG (www.microbesng.uk) was supported by the BBSRC (grant number BB/L024209/1). | en |
dc.description.abstract | Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen. | |
dc.format.extent | 7 | |
dc.format.extent | 1388646 | |
dc.language.iso | eng | |
dc.relation.ispartof | Veterinary Microbiology | en |
dc.subject | Diagnostic | en |
dc.subject | mPCR | en |
dc.subject | Capsule typing | en |
dc.subject | A. pleuropneumoniae | en |
dc.subject | Serovars | en |
dc.subject | QR Microbiology | en |
dc.subject | QH301 Biology | en |
dc.subject | DAS | en |
dc.subject.lcc | QR | en |
dc.subject.lcc | QH301 | en |
dc.title | Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Infection and Global Health Division | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.contributor.institution | University of St Andrews. Infection Group | en |
dc.identifier.doi | https://doi.org/10.1016/j.vetmic.2018.05.011 | |
dc.description.status | Peer reviewed | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.