Now showing items 1-10 of 52
Advanced light-sheet and structured illumination microscopy techniques for neuroscience and disease diagnosis
(University of St Andrews, 2016-11-30) - Thesis
Optical techniques for the investigation of a mechanical role for FRMD6/Willin in the Hippo signalling pathway
(University of St Andrews, 2019-06-24) - Thesis
The mammalian hippo signalling pathway controls cell proliferation and apoptosis via transcriptional co-activators YAP and TAZ, and as such is a key regulator of organ and tissue growth. Multiple cellular components ...
Tailoring optical fibers for cell transfection
(University of St Andrews, 2012-11-30) - Thesis
Optical transfection is a promising technique for the delivery of foreign genetic material into cells by transiently changing the permeability of the cell membrane. Of the different optical light sources that have been ...
Single cell transfection by laser-induced breakdown of an optically trapped gold nanoparticle
(SPIE, 2014-03-07) - Conference item
Cell selective introduction of therapeutic agents remains a challenging problem. Cavitation-based therapies including ultrasound-induced sonoporation and laser-induced optoporation have led the way for novel approaches to ...
Optical spectroscopic analysis for the discrimination of Extra-Virgin Olive Oil
(2016-11) - Journal article
We demonstrate the ability to discriminate between five brands of commercially available extra-virgin olive oil (EVOO) using Raman spectroscopy or fluorescence spectroscopy. Data was taken on both a ‘bulk optics’ free space ...
Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis
(2015-04-01) - Journal article
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. ...
Exploring the ultrashort pulse laser parameter space for membrane permeabilisation in mammalian cells.
(2012) - Journal article
The use of ultrashort femtosecond pulsed lasers to effect membrane permeabilisation and initiate both optoinjection and transfection of cells has recently seen immense interest. We investigate femtosecond laser-induced ...
Visualization of podocyte substructure with structured illumination microscopy (SIM) : a new approach to nephrotic disease
(2016-02-01) - Journal article
A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used ...
Imaging the cellular response to transient shear stress using time-resolved digital holography
(SPIE, 2014-02-26) - Conference item
Shear stress has been recognized as one of the biophysical methods by which to permeabilize plasma membranes of cells. In particular, high pressure transient hydrodynamic flows created by laser-induced cavitation have been ...
Wavefront corrected light sheet microscopy in turbid media
(2012-05) - Journal article
Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel ...