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dc.contributor.authorKillip, Marian Jane
dc.contributor.authorJackson, David
dc.contributor.authorPérez - Cidoncha, Maite
dc.contributor.authorFofor, Ervin
dc.contributor.authorRandall, Richard Edward
dc.date.accessioned2017-03-27T10:30:10Z
dc.date.available2017-03-27T10:30:10Z
dc.date.issued2017-03
dc.identifier.citationKillip , M J , Jackson , D , Pérez - Cidoncha , M , Fofor , E & Randall , R E 2017 , ' Single-cell studies of IFN-β promoter activation by wildtype and NS1-defective influenza A viruses ' , Journal of General Virology , vol. 98 , no. 3 , pp. 357-363 . https://doi.org/10.1099/jgv.0.000687en
dc.identifier.issn0022-1317
dc.identifier.otherPURE: 248203389
dc.identifier.otherPURE UUID: abc98f04-a928-4c6b-a800-36ec88faf670
dc.identifier.otherScopus: 85016959780
dc.identifier.otherORCID: /0000-0002-9304-6678/work/60427033
dc.identifier.otherWOS: 000399235600007
dc.identifier.urihttp://hdl.handle.net/10023/10527
dc.description.abstractDeletion or truncation of NS1, the principal interferon (IFN) antagonist of influenza viruses, leads to increased IFN induction during influenza virus infection. We have studied activation of the IFN induction cascade by both wildtype and NS1-defective viruses at the single-cell level using a cell line expressing GFP under the control of the IFN-β promoter and by examining MxA expression. The IFN-β promoter was not activated in all infected cells, even during NS1-defective virus infections. Loss of NS1 expression is therefore by itself insufficient to induce IFN in an infected cell, and factors besides NS1 expression status must dictate whether the IFN response is activated. The IFN response was efficiently stimulated in these cells following infection with other viruses; the differential IFN response we observe with influenza viruses is therefore not cell-specific, but is likely due to differences in the nature of the infecting virus particles and their subsequent replication.
dc.format.extent7
dc.language.isoeng
dc.relation.ispartofJournal of General Virologyen
dc.rightsCopyright 2017 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.en
dc.subjectInfluenza virusen
dc.subjectNS1en
dc.subjectInterferonen
dc.subjectIFNen
dc.subjectInnate immune responseen
dc.subjectInterferon antagonisten
dc.subjectQR180 Immunologyen
dc.subjectQR355 Virologyen
dc.subjectRB Pathologyen
dc.subjectNDASen
dc.subject.lccQR180en
dc.subject.lccQR355en
dc.subject.lccRBen
dc.titleSingle-cell studies of IFN-β promoter activation by wildtype and NS1-defective influenza A virusesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1099/jgv.0.000687
dc.description.statusPeer revieweden
dc.date.embargoedUntil2017-03-20


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