Now showing items 1-20 of 28

  • Adenovirus-mediated Cre deletion of floxed sequences in primary mouse cells is an efficient alternative for studies of gene deletion 

    Prost, Sandrine; Sheahan, Sheahan; Rannie, Dominic; Harrison, David (2001-08-15) - Journal article
    This study evaluates the utility of Cre-expressing adenovirus for deletion of floxed genes in primary cells using primary murine hepatocytes. Adenovirus infection was very efficient, even at very low MOI (>95% infection ...
  • Antitumour activity of the novel flavonoid oncamex in preclinical breast cancer models 

    Martínez-Pérez, Carlos; Ward, Carol; Turnbull, Arran K; Mullen, Peter; Cook, Graham; Meehan, James; Jarman, Edward J; Thomson, Patrick I T; Campbell, Colin J; McPhail, Donald; Harrison, David James; Langdon, Simon P (2016-04-13) - Journal article
    Background: The natural polyphenol myricetin induces cell cycle arrest and apoptosis in preclinical cancer models. We hypothesised that myricetin-derived flavonoids with enhanced redox properties, improved cell uptake and ...
  • ATG5 is essential for ATG8-dependent autophagy and mitochondrial homeostasis in Leishmania major 

    Williams, Roderick A. M.; Smith, Terry K.; Cull, Benjamin; Mottram, Jeremy C.; Coombs, Graham H. (2012-05-17) - Journal article
    Macroautophagy has been shown to be important for the cellular remodelling required for Leishmania differentiation. We now demonstrate that L. major contains a functional ATG12-ATG5 conjugation system, which is required ...
  • Changes in selective pressures associated with human population expansion may explain metabolic and immune related pathways enriched for signatures of positive selection 

    Vatsiou, Alexandra; Bazin, Eric; Gaggiotti, Oscar Eduardo (2016-07-21) - Journal article
    Background: The study of local adaptation processes is a very important research topic in the field of population genomics. There is a particular interest in the study of human populations because they underwent a process ...
  • Clinical and parasitological response to oral chloroquine and primaquine in uncomplicated human Plasmodium knowlesi infections 

    Daneshvar, Cyrus; Davis, Timothy M. E.; Cox Singh, Janet; Rafa'ee, Mohammad Z.; Zakaria, Siti K.; Divis, Paul C. S.; Singh, Balbir (2010-08-19) - Journal article
    Background: Plasmodium knowlesi is a cause of symptomatic and potentially fatal infections in humans. There are no studies assessing the detailed parasitological response to treatment of knowlesi malaria infections in man ...
  • Could molecular pathology testing in lung cancer be more cost effective? 

    Walsh, Kathy; Kheng, Yuan; Oniscu, Anca; Harrison, David James; Wallace, William (2016-10) - Journal article
    Aims: EGFR and ALK analysis is routinely undertaken prior to targeted treatment of non-squamous non-small cell lung carcinoma (NSCLC). Increasingly limited resources require molecular pathology services to be cost effective ...
  • Cytoadherence and virulence - the case of Plasmodium knowlesi malaria 

    Fatih, Farrah A.; Siner, Angela; Ahmed, Atique; Woon, Lu Chan; Craig, Alister G.; Singh, Balbir; Krishna, Sanjeev; Cox Singh, Janet (2012-02-03) - Journal article
    Background: Cytoadherence of infected red blood cells to brain endothelium is causally implicated in malarial coma, one of the severe manifestations of falciparum malaria. Cytoadherence is mediated by specific binding of ...
  • The effect of interleukin-1 receptor antagonist therapy on markers of inflammation in non-ST elevation acute coronary syndromes : the MRC-ILA Heart Study 

    Morton, Allison C; Rothman, Alexander M K; Greenwood, John P; Gunn, Julian; Chase, Alex; Clarke, Bernard; Hall, Alistair S; Fox, Keith; Foley, Claire; Banya, Winston; Wang, Duolao; Flather, Marcus D; Crossman, David C (2015-02-07) - Journal article
    Aims: Acute coronary syndromes (ACSs) are driven by inflammation within coronary plaque. Interleukin-1 (IL-1) has an established role in atherogenesis and the vessel-response to injury. ACS patients have raised serum markers ...
  • Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis 

    Freibert, Sven-A; Goldberg, Alina V.; Hacker, Christian; Molik, Sabine; Dean, Paul; Williams, Tom A.; Nakjang, Sirintra; Long, Shaojun; Sendra, Kacper; Bill, Eckhard; Heinz, Eva; Hirt, Robert P.; Lucocq, John Milton; Embley, T. Martin; Lill, Roland (2017-01-04) - Journal article
    Microsporidians are a diverse group of obligate intracellular parasites that have minimized their genome content and simplified their sub-cellular structures by reductive evolution. Functional studies are limited because ...
  • Global network analysis of drug tolerance, mode of action and virulence in methicillin-resistant S. aureus 

    Overton, Ian M.; Graham, Shirley; Gould, Katherine A.; Hinds, Jason; Botting, Catherine H.; Shirran, Sally; Barton, Geoffrey J.; Coote, Peter J. (2011-05-12) - Journal article
    Background: Staphylococcus aureus is a major human pathogen and strains resistant to existing treatments continue to emerge. Development of novel treatments is therefore important. Antimicrobial peptides represent a source ...
  • Human tissue in systems medicine 

    Caie, Peter David; Schuur, Klaas; Oniscu, Anca; Mullen, Peter; Reynolds, Paul Andrew; Harrison, David James (2013-12) - Journal item
    Histopathology, the examination of an architecturally artefactual, two dimensional, static image remains a potent tool allowing diagnosis and empirical expectation of prognosis. Considerable optimism exists that the advent ...
  • Human urinary exosomes in bladder cancer patients : properties, concentrations and possible clinical application 

    Riches, Andrew Clive; Powis, Simon John; Mullen, Peter; Harrison, David James; Hacker, Christian; Lucocq, John Milton; Bowness, James Simeon; Chapman, Alex; Cameron, Ruth; McLornan, Liz; Chinn, David John; Leung, Steve (2015-12-11) - Journal article
    OBJECTIVE: High grade bladder cancer is extremely aggressive. Early detection is thus an important challenge. De- velopment of non-invasive diagnostic tools particularly using urine samples could be of importance in the ...
  • Identification of novel inhibitors of the type I interferon induction pathway using cell-based high-throughput screening 

    Gage, Zoe Olivia; Vasou, Andri; Gray, David; Randall, Richard Edward; Adamson, Catherine Sarah (2016-10) - Journal article
    Production of type I interferon (IFN) is an essential component of the innate immune response against invading pathogens. However, its production must be tightly regulated to avoid harmful effects. Compounds that modulate ...
  • Investigation of metabolites for estimating blood deposition time 

    Lech, Karolina; Liu, Fan; Davies, Sarah K.; Ackermann, Katrin; Ang, Joo Ern; Middleton, Benita; Revell, Victoria L.; Raynaud, Florence J.; Hoveijn, Igor; Hut, Roelof A.; Skene, Debra J.; Kayser, Manfred (2017-08-05) - Journal article
    Trace deposition timing reflects a novel concept in forensic molecular biology involving the use of rhythmic biomarkers for estimating the time within a 24-h day/night cycle a human biological sample was left at the crime ...
  • Knowlesi malaria in Vietnam 

    Cox Singh, Janet (2009-11-26) - Journal article
    The simian malaria parasite Plasmodium knowlesi is transmitted in the forests of Southeast Asia. Symptomatic zoonotic knowlesi malaria in humans is widespread in the region and is associated with a history of spending time ...
  • Micro-geographic risk factors for malarial infection 

    Myers, Ward P.; Myers, Andrea P.; Cox Singh, Janet; Lau, Hui C.; Mokuai, Benny; Malley, Richard (2009-02-13) - Journal article
    Background: Knowledge of geography is integral to the study of insect-borne infectious disease such as malaria. This study was designed to evaluate whether geographic parameters are associated with malarial infection in ...
  • Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections 

    Lee, Kim-Sung; Cox Singh, Janet; Singh, Balbir (2009-04-21) - Journal article
    Background: Human infections with Plasmodium knowlesi, a simian malaria parasite, are more common than previously thought. They have been detected by molecular detection methods in various countries in Southeast Asia, where ...
  • Next-generation pathology 

    Caie, Peter D; Harrison, David J (Humana Press, 2016) - Book item
    The field of pathology is rapidly transforming from a semiquantitative and empirical science toward a big data discipline. Large data sets from across multiple omics fields may now be extracted from a patient's tissue ...
  • Novel histopathologic feature identified through image analysis augments stage II colorectal cancer clinical reporting 

    Caie, Peter David; Zhou, Ying; Turnbull, Arran; Oniscu, Anca; Harrison, David James (2016-06-15) - Journal article
    A number of candidate histopathologic factors show promise in identifying stage II colorectal cancer (CRC) patients at a high risk of disease-specific death, however they can suffer from low reproducibility and none have ...
  • One For All? Hitting multiple Alzheimer’s Disease targets with one drug 

    Hughes, Rebecca E.; Nikolic, Katarina; Ramsay, Rona Ruth (2016-04-25) - Journal item
    Alzheimer’s Disease is a complex and multifactorial disease for which the mechanism is still not fully understood. As new insights into disease progression are discovered, new drugs must be designed to target those aspects ...