Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity
Date
10/02/2012Author
Grant ID
BBS/B/14426
BB/G011400/1
BBS/B/14426
BB/G011400/1
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Show full item recordAbstract
The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endo-nucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a proto-spacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.
Citation
Zhang , J , Rouillon , C , Kerou , M , Reeks , J , Brugger , K , Graham , S , Reimann , J , Cannone , G , Liu , H , Albers , S-V , Naismith , J H , Spagnolo , L & White , M F 2012 , ' Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity ' , Molecular Cell , vol. 45 , no. 3 , pp. 303-313 . https://doi.org/10.1016/j.molcel.2011.12.013
Publication
Molecular Cell
Status
Peer reviewed
ISSN
1097-2765Type
Journal article
Rights
(c)2012 Elsevier Inc. This article is available under an Elsevier user license which allows re-use for non-commercial purposes. See http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-license
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