Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9
Date
27/06/2014Metadata
Show full item recordAbstract
Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use the RNA-guided Cas9 nuclease to efficiently generate knockouts without selection, and to introduce point mutations and epitope tags into the T. gondii genome. These methods will streamline the functional analysis of parasite genes and enable high-throughput engineering of their genomes.
Citation
Sidik , S M , Hackett , C G , Tran , F , Westwood , N J & Lourido , S 2014 , ' Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9 ' , PLoS One , vol. 9 , no. 6 , e100450 . https://doi.org/10.1371/journal.pone.0100450
Publication
PLoS One
Status
Peer reviewed
ISSN
1932-6203Type
Journal article
Description
This work was supported in part by NIH grant 1DP5OD017892 to S.LCollections
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