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Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/418
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Title: A study of the behaviour and interactions of the novel FERM protein Willin
Authors: Herron, Lissa Rocha
Supervisors: Gunn-Moore, Frank J.
Guild, Simon
Keywords: Willin
FERM
Merlin
Ezrin
Radixin
Moesin
Neurofascin
L1
Issue Date: 2008
Abstract: Willin is a novel member of the Four-point-one Ezrin Radixin Moesin (FERM) protein superfamily, containing an N-terminal FERM domain most like the Ezrin-Radixin-Moesin (ERM) family but also the closely related protein Merlin. Willin was initially discovered as a yeast two-hybrid binding partner of neurofascin155, and this interaction has now been confirmed by both co-localisation studies and the use of two different biochemical methods. Like neurofascin155, Willin also localises to detergent resistant membranes, and like the ERM family, it is able to bind to phospholipids. The expression of Willin appears to be toxic as the production of cell-lines stably expressing Willin proved to be not possible and this appears to be because it induces apoptosis in cultured cells. This is a proliferation control function consistent with the suggestion that Willin is the human homologue of the Drosophila tumour suppressor ‘Expanded’. Three antibodies to Willin were also characterised and a novel splice variant, Willin2, subcloned into a GFP-tagged plasmid for comparison with the original form.
URI: http://hdl.handle.net/10023/418
Type: Thesis
Publisher: University of St Andrews
Appears in Collections:Biology Theses



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