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Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/3286
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Title: Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDOR
Authors: Reginsson, Gunnar Widtfeldt
Shelke, Sandip
Rouillon, Christophe
White, Malcolm F
Sigurdsson, Snorri T
Schiemann, Olav
Keywords: Binding
PULSED ELECTRON
Nucleic-acids
Conformation
Lactose operon
Distance measurements
Operator
EPR spectroscopy
Electron-paramagnetic-resonance
LAC repressor
QH426 Genetics
QD Chemistry
Issue Date: Jan-2013
Citation: Reginsson , G W , Shelke , S , Rouillon , C , White , M F , Sigurdsson , S T & Schiemann , O 2013 , ' Protein-induced changes in DNA structure and dynamics observed with noncovalent site-directed spin-labeling and PELDOR ' Nucleic Acids Research , vol 41 , no. 1 , e11 .
Abstract: Site-directed spin labeling and pulsed electron–electron double resonance (PELDOR or DEER) have previously been applied successfully to study the structure and dynamics of nucleic acids. Spin labeling nucleic acids at specific sites requires the covalent attachment of spin labels, which involves rather complicated and laborious chemical synthesis. Here, we use a noncovalent label strategy that bypasses the covalent labeling chemistry and show that the binding specificity and efficiency are large enough to enable PELDOR or DEER measurements in DNA duplexes and a DNA duplex bound to the Lac repressor protein. In addition, the rigidity of the label not only allows resolution of the structure and dynamics of oligonucleotides but also the determination of label orientation and protein-induced conformational changes. The results prove that this labeling strategy in combination with PELDOR has a great potential for studying both structure and dynamics of oligonucleotides and their complexes with various ligands.
Version: Publisher PDF
Status: Peer reviewed
URI: http://hdl.handle.net/10023/3286
DOI: http://dx.doi.org/10.1093/nar/gks817
ISSN: 0305-1048
Type: Journal article
Rights: © The Author(s) 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Appears in Collections:Biomedical Sciences Research Complex (BSRC) Research
Biology Research
University of St Andrews Research



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