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Title: Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples
Authors: Canetta, Elisabetta
Mazilu, Michael
De Luca, Anna Chiara
Carruthers, Antonia Erika
Dholakia, Kishan
Neilson, Sam
Sargeant, Harry
Briscoe, Tina
Herrington, C Simon
Riches, Andrew Clive
Keywords: Raman spectroscopy
Modulation spectroscopy
Bladder cancer
RC0254 Neoplasms. Tumors. Oncology (including Cancer)
QC Physics
Issue Date: Mar-2011
Citation: Canetta , E , Mazilu , M , De Luca , A C , Carruthers , A E , Dholakia , K , Neilson , S , Sargeant , H , Briscoe , T , Herrington , C S & Riches , A C 2011 , ' Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples ' Journal of Biomedical Optics , vol 16 , no. 3 , 037002 .
Abstract: Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows thatMRS allows discrimination between Raman spectra of SV-HUC-1 andMGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h.We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples.
Version: Publisher PDF
Status: Peer reviewed
URI: http://hdl.handle.net/10023/3002
DOI: http://dx.doi.org/10.1117/1.3556722
ISSN: 1083-3668
Type: Journal article
Rights: Copyright 2011 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/1.3556722
Appears in Collections:University of St Andrews Research
Medicine Research
Physics & Astronomy Research



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