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Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/2999
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Title: Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo
Authors: Stramer, Brian
Moreira, Severina
Millard, Tom
Evans, Iwan
Huang, Chieh-Yin
Sabet, Ola
Milner, Martin John
Dunn, Graham
Martin, Paul
Wood, Will
Keywords: QH301 Biology
Issue Date: 17-May-2010
Citation: Stramer , B , Moreira , S , Millard , T , Evans , I , Huang , C-Y , Sabet , O , Milner , M J , Dunn , G , Martin , P & Wood , W 2010 , ' Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo ' Journal of Cell Biology , vol 189 , no. 4 , pp. 681-689 .
Abstract: Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
Version: Publisher PDF
Description: P. Martin and W. Wood contributed equally to this paper
Status: Peer reviewed
URI: http://hdl.handle.net/10023/2999
http://jcb.rupress.org/content/189/4/681.abstract
DOI: http://dx.doi.org/10.1083/jcb.200912134
ISSN: 0021-9525
Type: Journal article
Rights: © 2010 Stramer et al. This article is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
Appears in Collections:University of St Andrews Research
Biology Research



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