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Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/2996
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Title: Conservation of a crystallographic interface suggests a role for beta-sheet augmentation in influenza virus NS1 multifunctionality
Authors: Kerry, Philip Stephen
Long, E
Taylor, Margaret Alexandra
Russell, Rupert James Martin
Keywords: Effector domains
Influenza virus
Virulence factors
NS1
β-sheet augmentation
QR355 Virology
Issue Date: 1-Aug-2011
Citation: Kerry , P S , Long , E , Taylor , M A & Russell , R J M 2011 , ' Conservation of a crystallographic interface suggests a role for beta-sheet augmentation in influenza virus NS1 multifunctionality ' Acta Crystallographica. Section F, Structural biology and crystallization communications , vol 67 , no. 8 , pp. 858-861 .
Abstract: The effector domain (ED) of the influenza virus virulence factor NS1 is capable of interaction with a variety of cellular and viral targets, although regulation of these events is poorly understood. Introduction of a W187A mutation into the ED abolishes dimer formation; however, strand-strand interactions between mutant NS1 ED monomers have been observed in two previous crystal forms. A new condition for crystallization of this protein [0.1 M Bis-Tris pH 6.0, 0.2 M NaCl, 22%(w/v) PEG 3350, 20 mM xylitol] was discovered using the hanging-drop vapour-diffusion method. Diffraction data extending to 1.8 Å resolution were collected from a crystal grown in the presence of 40 mM thieno[2,3-b]pyridin-2-ylmethanol. It was observed that there is conservation of the strand-strand interface in crystals of this monomeric NS1 ED in three different space groups. This observation, coupled with conformational changes in the interface region, suggests a potential role for [beta]-sheet augmentation in NS1 function.
Version: Publisher PDF
Description: This research was supported by grants from the Medical Research Council (MRC) and the Scottish Funding Council (SFC).
Status: Peer reviewed
URI: http://hdl.handle.net/10023/2996
DOI: http://dx.doi.org/10.1107/S1744309111019312
ISSN: 1744-3091
Type: Journal article
Rights: Copyright © Kerry et al. 2011. This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
Appears in Collections:University of St Andrews Research
Biology Research
Biomedical Sciences Research Complex (BSRC) Research



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