Conservation of a crystallographic interface suggests a role for beta-sheet augmentation in influenza virus NS1 multifunctionality
Date
01/08/2011Metadata
Show full item recordAbstract
The effector domain (ED) of the influenza virus virulence factor NS1 is capable of interaction with a variety of cellular and viral targets, although regulation of these events is poorly understood. Introduction of a W187A mutation into the ED abolishes dimer formation; however, strand-strand interactions between mutant NS1 ED monomers have been observed in two previous crystal forms. A new condition for crystallization of this protein [0.1 M Bis-Tris pH 6.0, 0.2 M NaCl, 22%(w/v) PEG 3350, 20 mM xylitol] was discovered using the hanging-drop vapour-diffusion method. Diffraction data extending to 1.8 Å resolution were collected from a crystal grown in the presence of 40 mM thieno[2,3-b]pyridin-2-ylmethanol. It was observed that there is conservation of the strand-strand interface in crystals of this monomeric NS1 ED in three different space groups. This observation, coupled with conformational changes in the interface region, suggests a potential role for [beta]-sheet augmentation in NS1 function.
Citation
Kerry , P S , Long , E , Taylor , M A & Russell , R J M 2011 , ' Conservation of a crystallographic interface suggests a role for beta-sheet augmentation in influenza virus NS1 multifunctionality ' , Acta Crystallographica. Section F, Structural biology and crystallization communications , vol. 67 , no. 8 , pp. 858-861 . https://doi.org/10.1107/S1744309111019312
Publication
Acta Crystallographica. Section F, Structural biology and crystallization communications
Status
Peer reviewed
ISSN
1744-3091Type
Journal article
Rights
Copyright © Kerry et al. 2011. This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
Description
This research was supported by grants from the Medical Research Council (MRC) and the Scottish Funding Council (SFC).Collections
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