Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei
Abstract
The biological membranes of Trypanosonza brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification of similar to 500 individual molecular phospholipids species from total lipid extracts of cultured bloodstream and procyclic form T. brucei. Various molecular species of all of the major subclasses of glycerophospholipids were identified including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol as well as phosphatidic acid, phosphatidylglycerol and cardolipin, and the sphingolipids sphingomyelin, inositol phosphoceramide and ethanolamine phosphoceramide. The lipidomic data obtained in this study will aid future biochemical phenotyping of either genetically or chemically manipulated commonly used bloodstream and procyclic strains of Trypanosoma brucei. Hopefully this will allow a greater understanding of the bizarre world of lipids in this important human pathogen.
Citation
Richmond , G S , Gibellini , F , Young , S A , Major , L , Denton , H , Lilley , A & Smith , T K 2010 , ' Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei ' , Parasitology , vol. 137 , no. 9 , pp. 1357-1392 . https://doi.org/10.1017/S0031182010000715
Publication
Parasitology
Status
Peer reviewed
ISSN
0031-1820Type
Journal article
Rights
(c)2010 Cambridge University Press
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