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Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/2117
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Title: Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei
Authors: Richmond, Gregory S.
Gibellini, Federica
Young, Simon A.
Major, Louise
Denton, Helen
Lilley, Alison
Smith, Terry K
Keywords: Phospholipid
Trypanosonza brucei mass spectrometry
lipidomics
Gpi-anchored proteins
De-novo synthesis
Kennedy pathway
Sphingolipid synthesisv
African trypanosomes
Myristate exchange
Mass-spectrometry
Culture forms
Phospholipids
Metabolism
QH301 Biology
QD Chemistry
Issue Date: Aug-2010
Citation: Richmond , G S , Gibellini , F , Young , S A , Major , L , Denton , H , Lilley , A & Smith , T K 2010 , ' Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei ' Parasitology , vol 137 , no. 9 , pp. 1357-1392 .
Abstract: The biological membranes of Trypanosonza brucei contain a complex array of phospholipids that are synthesized de novo from precursors obtained either directly from the host, or as catabolised endocytosed lipids. This paper describes the use of nanoflow electrospray tandem mass spectrometry and high resolution mass spectrometry in both positive and negative ion modes, allowing the identification of similar to 500 individual molecular phospholipids species from total lipid extracts of cultured bloodstream and procyclic form T. brucei. Various molecular species of all of the major subclasses of glycerophospholipids were identified including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol as well as phosphatidic acid, phosphatidylglycerol and cardolipin, and the sphingolipids sphingomyelin, inositol phosphoceramide and ethanolamine phosphoceramide. The lipidomic data obtained in this study will aid future biochemical phenotyping of either genetically or chemically manipulated commonly used bloodstream and procyclic strains of Trypanosoma brucei. Hopefully this will allow a greater understanding of the bizarre world of lipids in this important human pathogen.
Version: Publisher PDF
Status: Peer reviewed
URI: http://hdl.handle.net/10023/2117
DOI: http://dx.doi.org/10.1017/S0031182010000715
ISSN: 0031-1820
Type: Journal article
Rights: (c)2010 Cambridge University Press
Appears in Collections:University of St Andrews Research
Biology Research
Biomedical Sciences Research Complex (BSRC) Research



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