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Title: Crystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia coli
Authors: Bushell, Simon R.
Lou, Hubing
Wallat, Gregor D.
Beis, Konstantinos
Whitfield, Chris
Naismith, James H.
Keywords: Wzi
Polysaccharide capsules
Escherichia coli
Outer-membrane
Klebsiella-pneumoniae
Colanic acid
Protein
Polysaccharide
Expression
Translocation
Biosythesis
Organization
Crystals
QD Chemistry
Issue Date: Dec-2010
Citation: Bushell , S R , Lou , H , Wallat , G D , Beis , K , Whitfield , C & Naismith , J H 2010 , ' Crystallization and preliminary diffraction analysis of Wzi, a member of the capsule export and assembly pathway in Escherichia coli ' Acta Crystallographica. Section F, Structural biology and crystallization communications , vol 66 , no. 12 , pp. 1621-1625 .
Abstract: External polysaccharide capsules provide a physical barrier that is employed by many species of bacteria for the purposes of host evasion and persistence. Wzi is a 53 kDa outer membrane beta-barrel protein that is thought to play a role in the attachment of group 1 capsular polysaccharides to the cell surface. The purification and crystallization of an Escherichia coli homologue of Wzi is reported and diffraction data from native and selenomethionine-incorporated protein crystals are presented. Crystals of C-terminally His(6)-tagged Wzi diffracted to 2.8 A resolution. Data processing showed that the crystals belonged to the orthorhombic space group C222, with unit-cell parameters a = 128.8, b = 152.8, c = 94.4 A, alpha = beta = gamma = 90 degrees. A His-tagged selenomethionine-containing variant of Wzi has also been crystallized in the same space group and diffraction data have been recorded to 3.8 A resolution. Data processing shows that the variant crystal has similar unit-cell parameters to the native crystal.
Version: Publisher PDF
Description: Supported by a grant from The Wellcome Trust (081862/Z/06/Z)
Status: Peer reviewed
URI: http://hdl.handle.net/10023/1800
DOI: http://dx.doi.org/10.1107/S1744309110040546
ISSN: 1744-3091
Type: Journal article
Rights: This is an open access article distributed under a Creative Commons licence (http://creativecommons.org/licenses/by/2.0/uk/legalcode) which permits you to reuse and copy the article provided the original authors and source are cited.
Appears in Collections:University of St Andrews Research
Chemistry Research
Biomedical Sciences Research Complex (BSRC) Research



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