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Please use this identifier to cite or link to this item: http://hdl.handle.net/10023/1148
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Title: The N-terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism
Authors: Van Knippenberg, Ingeborg Christine
Carlton-Smith, Charles
Elliott, Richard Michael
Keywords: QR Microbiology
Issue Date: Apr-2010
Citation: Van Knippenberg , I C , Carlton-Smith , C & Elliott , R M 2010 , ' The N-terminus of Bunyamwera orthobunyavirus NSs protein is essential for interferon antagonism ' Journal of General Virology , vol 91 , no. 8 , pp. 2002-2006 .
Abstract: Bunyamwera virus NSs protein is involved in the inhibition of cellular transcription and the interferon (IFN) response, and it interacts with the Med8 component of Mediator. A spontaneous mutant of a recombinant NSs-deleted Bunyamwera virus (rBUNdelNSs2) was identified and characterized. This mutant virus, termed mBUNNSs22, expresses a 21 aa N-terminally truncated form of NSs. Like rBUNdelNSs2, mBUNNSs22 is attenuated in IFN-deficient cells, and to a greater extent in IFN-competent cells. Both rBUNdelNSs2 and mBUNNSs22 are potent IFN inducers and their growth can be rescued by depleting cellular IRF3. Strikingly, despite encoding an NSs protein that contains the Med8 interaction domain, mBUNNSs22 fails to block RNA polymerase II activity during infection. Overall, our data suggest that both the interaction of NSs with Med8 and a novel unidentified function of the NSs N-terminus, seem necessary for Bunyamwera virus to counteract host antiviral responses.
Version: Publisher PDF
Description: This work is supported by UK MRC and BBRC
Status: Peer reviewed
URI: http://hdl.handle.net/10023/1148
DOI: http://dx.doi.org/10.1099/vir.0.021774-0
ISSN: 0022-1317
Type: Journal article
Rights: This article is published by the Society for General Microbiology under the Open Option which allows reuse for non commercial purposes.
Appears in Collections:University of St Andrews Research
Biology Research
Biomedical Sciences Research Complex (BSRC) Research



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