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Title: Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells : Implications for luteolysis
Authors: Dickinson, Rachel E
Stewart, Alan J.
Myers, Michelle
Millar, Robert P
Duncan, W Colin
Keywords: RC Internal medicine
Issue Date: Jun-2009
Citation: Dickinson , R E , Stewart , A J , Myers , M , Millar , R P & Duncan , W C 2009 , ' Differential expression and functional characterization of luteinizing hormone receptor splice variants in human luteal cells : Implications for luteolysis ' Endocrinology , vol 150 , no. 6 , pp. 2873-2881 .
Abstract: The human LH receptor (LHR) plays a key role in luteal function and the establishment of pregnancy through its interaction with the gonadotropins LH and human chorionic gonadotropin. We previously identified four splice variants of the LHR in human luteinized granulosa cells (LGCs) and corpora lutea (CL). Real-time quantitative PCR revealed that expression of the full-length LHR (LHRa) and the most truncated form (LHRd) changed significantly in CL harvested at different stages of the ovarian cycle (P < 0.01, ANOVA). LHRa expression was reduced in the late luteal CL (P<0.05). Conversely, an increase in LHRd expression was observed in the late luteal CL (P<0.01). Chronic manipulation of human chorionic gonadotropin in LGC primary cultures supported the in vivo findings. LHRd encodes a protein lacking the transmembrane and carboxyl terminal domains. COS-7 cells expressing LHRd were unable to produce cAMP in response to LH stimulation. COS-7 cells coexpressing LHRd and LHRa also failed to generate cAMP in response to LH, suggesting that this truncated form has a negative effect on the signaling of LHRa. Immunofluorescence staining ofLGC and COS-7 cells implied that there is a reduction in cell surface expression ofLHRa when LHRd is present. Overall, these results imply expression of LHR splice variants is regulated in the human CL. Furthermore, during functional luteolysis a truncated variant could modulate the cell surface expression and activity of full-length LHR.
Version: Publisher PDF
Status: Peer reviewed
URI: http://hdl.handle.net/10023/1049
http://endo.endojournals.org/cgi/content/full/150/6/2873
DOI: http://dx.doi.org/10.1210/en.2008-1382
ISSN: 0013-7227
Type: Journal article
Rights: Copyright © 2009 by The Endocrine Society. Publisher version deposited in accordance with publisher's policy.
Appears in Collections:Institute of Behavioural and Neural Sciences Research
University of St Andrews Research
Medicine Research
Biomedical Sciences Research Complex (BSRC) Research



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