http://hdl.handle.net/10023/183 2013-05-26T02:42:01Z 2013-05-26T02:42:01Z Harris, Danielle V. http://hdl.handle.net/10023/3463 2013-04-03T14:34:48Z 2012-06-20T00:00:00Z

Abstract: Passive acoustic monitoring has been used to investigate many aspects of marine mammal ecology, although methods to estimate absolute abundance and density using acoustic data have only been developed in recent years. The instrument configuration in an acoustic survey determines which abundance estimation methods can be used. Sparsely distributed arrays of instruments are useful because wide geographic areas can be covered. However, instrument spacing in sparse arrays is such that the same vocalisation will not be detected on multiple instruments, excluding the use of some abundance estimation methods. The aim of this thesis was to explore cetacean abundance and density estimation using novel sparse array datasets, applying existing methods where possible, or developing new approaches. The wealth of data collected by sparse arrays was demonstrated by analysing a 10-year dataset collected by the U.S. Navy’s Sound Surveillance System in the north-east Atlantic. Spatial and temporal patterns of blue (Balaenoptera musculus) and fin whale (Balaenoptera physalus) vocal activity were investigated using generalised additive models. Distance sampling-based methods were applied to fin whale calls recorded by an array of Ocean Bottom Seismometers in the north-east Atlantic. Estimated call density was 993 calls/1000 km².hr⁻¹ (CV: 0.39). Animal density could not be estimated because the call rate was unknown. Further development of the call localisation method is required so the current density estimate may be biased. Furthermore, analysing a single day of data resulted in a high variance estimate. Finally, a new simulation-based method developed to estimate density from single hydrophones was applied to blue whale calls recorded in the northern Indian Ocean. Estimated call density was 3 calls/1000 km².hr⁻¹ (CV: 0.17). Again, density of whales could not be estimated as the vocalisation rate was unknown. Lack of biological knowledge poses the greatest limitation to abundance and density estimation using acoustic data.

2012-06-20T00:00:00Z Harris, Danielle V. Passive acoustic monitoring has been used to investigate many aspects of marine mammal ecology, although methods to estimate absolute abundance and density using acoustic data have only been developed in recent years. The instrument configuration in an acoustic survey determines which abundance estimation methods can be used. Sparsely distributed arrays of instruments are useful because wide geographic areas can be covered. However, instrument spacing in sparse arrays is such that the same vocalisation will not be detected on multiple instruments, excluding the use of some abundance estimation methods. The aim of this thesis was to explore cetacean abundance and density estimation using novel sparse array datasets, applying existing methods where possible, or developing new approaches. The wealth of data collected by sparse arrays was demonstrated by analysing a 10-year dataset collected by the U.S. Navy’s Sound Surveillance System in the north-east Atlantic. Spatial and temporal patterns of blue (Balaenoptera musculus) and fin whale (Balaenoptera physalus) vocal activity were investigated using generalised additive models. Distance sampling-based methods were applied to fin whale calls recorded by an array of Ocean Bottom Seismometers in the north-east Atlantic. Estimated call density was 993 calls/1000 km².hr⁻¹ (CV: 0.39). Animal density could not be estimated because the call rate was unknown. Further development of the call localisation method is required so the current density estimate may be biased. Furthermore, analysing a single day of data resulted in a high variance estimate. Finally, a new simulation-based method developed to estimate density from single hydrophones was applied to blue whale calls recorded in the northern Indian Ocean. Estimated call density was 3 calls/1000 km².hr⁻¹ (CV: 0.17). Again, density of whales could not be estimated as the vocalisation rate was unknown. Lack of biological knowledge poses the greatest limitation to abundance and density estimation using acoustic data. Arriola Ortiz, Aline http://hdl.handle.net/10023/895 2010-08-05T11:05:58Z 2010-01-01T00:00:00Z

Abstract: This thesis examines fatty acid (FA) and polychlorinated biphenyl (PCB) dynamics in a marine top predator, the grey seal (Halichoerus grypus,) and their transfer during lactation from mother to offspring. It examines regional and annual variations in FA composition and PCB loads, and also how the physical and chemical characteristics of these molecules (e.g. their polarity and size) can affect the rates of accumulation, mobilization and transfer of specific FAs or PCBs. Two UK grey seal colonies (North Rona (NR) and Isle of May (IOM) were studied during three consecutive years (1996-1998 and 2004-2006). Lactating grey seals and their pups were repeatedly captured during the lactation period and sampled for blubber, serum and milk and analysed for FAs and PCBs. Overall, the two colonies were clearly distinguished from each other, suggesting that the main prey species had different FA composition, and possibly that the seals from these colonies had different diets . These differences are probably a direct consequence of differences in prey community structure in the two regions where seals from these two colonies are thought to feed. Within each colony, annual differences could be detected between some years but not between others. During 1996-98, IOM seals showed a clear change in their FA profiles while NR seals did not. In contrast, during 2004-2006 NR seals showed a clear change while IOM seals did not. The changes observed in IOM during 1996-1998 are consistent with the large-scale regime shift that occurred in the North Sea during the 1990‟s. The relative proportions of each FA that were mobilized from blubber and transferred to the milk during lactation were very similar between colonies, and could be explained to a large degree by their physico-chemical properties. For a given carbon chain length the mobilization increased with increasing number of double bonds; and for a given number of double bonds the mobilization decrease with increasing carbon chain length. However, the mobilization also appeared to be influenced by the specific nutritional requirements of the growing pups. For instance, FAs that are considered essential for pup development or efficient energy storage (e.g. saturated FAs) were more highly mobilised than expected. This selectivity was also reflected in the FA composition of the different body compartments (maternal blubber and milk, pup blubber) that persisted throughout lactation. These changes were also similar between the colonies. Colonies could also be clearly distinguished by their blubber PCB profiles. IOM seals had higher total concentrations on average than NR seals (1327.9 vs. 680.2 ng/g lipid in 2005 and 1199.7 vs. 819.0 ng/g lipid in 2006). IOM seals also had higher total amounts in both years (79.2 vs. 38.0 mg in 2005 and 61.7 vs. 53.4 mg in 2006). One of the main differences between colonies was that females from IOM had higher concentrations of highly chlorinated congeners than NR seals.PCB concentrations in blubber increased towards the end of lactation. Serum and milk PCB concentrations also increased rapidly, especially for the highly chlorinated congeners. These results were consistent with other studies showing the increase in concentrations as a result of lipid loss. Serum concentrations stayed constant during the first part of lactation and increased at late lactation. This was also observed in milk PCB concentrations. The changes in the PCB profiles in the three body compartments were very similar between colonies. However IOM seals always had higher total concentrations of PCBs in all of the body compartments. The concentrations of individual congeners relative to PCB-153 showed that blubber contained higher proportions of the highly chlorinated PCBs relative to other tissues. There were no clear changes in these proportions in blubber during lactation, but the relative proportions of highly chlorinated PCB In serum and milk increased throughout lactation while the less chlorinated PCBs stayed constant. The highly chlorinated PCBs were found in lower concentration in the milk compared to the less chlorinated compounds suggesting a selective release from blubber to blood and a selective transfer of PCBs to the milk.

2010-01-01T00:00:00Z Arriola Ortiz, Aline This thesis examines fatty acid (FA) and polychlorinated biphenyl (PCB) dynamics in a marine top predator, the grey seal (Halichoerus grypus,) and their transfer during lactation from mother to offspring. It examines regional and annual variations in FA composition and PCB loads, and also how the physical and chemical characteristics of these molecules (e.g. their polarity and size) can affect the rates of accumulation, mobilization and transfer of specific FAs or PCBs. Two UK grey seal colonies (North Rona (NR) and Isle of May (IOM) were studied during three consecutive years (1996-1998 and 2004-2006). Lactating grey seals and their pups were repeatedly captured during the lactation period and sampled for blubber, serum and milk and analysed for FAs and PCBs. Overall, the two colonies were clearly distinguished from each other, suggesting that the main prey species had different FA composition, and possibly that the seals from these colonies had different diets . These differences are probably a direct consequence of differences in prey community structure in the two regions where seals from these two colonies are thought to feed. Within each colony, annual differences could be detected between some years but not between others. During 1996-98, IOM seals showed a clear change in their FA profiles while NR seals did not. In contrast, during 2004-2006 NR seals showed a clear change while IOM seals did not. The changes observed in IOM during 1996-1998 are consistent with the large-scale regime shift that occurred in the North Sea during the 1990‟s. The relative proportions of each FA that were mobilized from blubber and transferred to the milk during lactation were very similar between colonies, and could be explained to a large degree by their physico-chemical properties. For a given carbon chain length the mobilization increased with increasing number of double bonds; and for a given number of double bonds the mobilization decrease with increasing carbon chain length. However, the mobilization also appeared to be influenced by the specific nutritional requirements of the growing pups. For instance, FAs that are considered essential for pup development or efficient energy storage (e.g. saturated FAs) were more highly mobilised than expected. This selectivity was also reflected in the FA composition of the different body compartments (maternal blubber and milk, pup blubber) that persisted throughout lactation. These changes were also similar between the colonies. Colonies could also be clearly distinguished by their blubber PCB profiles. IOM seals had higher total concentrations on average than NR seals (1327.9 vs. 680.2 ng/g lipid in 2005 and 1199.7 vs. 819.0 ng/g lipid in 2006). IOM seals also had higher total amounts in both years (79.2 vs. 38.0 mg in 2005 and 61.7 vs. 53.4 mg in 2006). One of the main differences between colonies was that females from IOM had higher concentrations of highly chlorinated congeners than NR seals.PCB concentrations in blubber increased towards the end of lactation. Serum and milk PCB concentrations also increased rapidly, especially for the highly chlorinated congeners. These results were consistent with other studies showing the increase in concentrations as a result of lipid loss. Serum concentrations stayed constant during the first part of lactation and increased at late lactation. This was also observed in milk PCB concentrations. The changes in the PCB profiles in the three body compartments were very similar between colonies. However IOM seals always had higher total concentrations of PCBs in all of the body compartments. The concentrations of individual congeners relative to PCB-153 showed that blubber contained higher proportions of the highly chlorinated PCBs relative to other tissues. There were no clear changes in these proportions in blubber during lactation, but the relative proportions of highly chlorinated PCB In serum and milk increased throughout lactation while the less chlorinated PCBs stayed constant. The highly chlorinated PCBs were found in lower concentration in the milk compared to the less chlorinated compounds suggesting a selective release from blubber to blood and a selective transfer of PCBs to the milk. Boehme, Lars http://hdl.handle.net/10023/687 2012-07-12T08:19:16Z 2008-11-27T00:00:00Z

Abstract: In this thesis, I describe large-scale and small-scale features of the Antarctic Circumpolar Current (ACC) by merging conductivity-temperature-depth (CTD) data obtained by novel animal-borne sensors with data obtained by more conventional means. Twenty-one CTD-Satellite Relay Data Loggers (CTD-SRDLs) were attached to Southern elephant seals (Mirounga leonina) on South Georgia in 2004 and 2005. This was part of a larger international study (Southern Elephant Seals as Oceanographic Samplers; SEaOS), in which I played a major role in developing the oceanographic approach used to integrate physical data from a range of sources, and the means to link biological findings to oceanographic parameters. The development of animal-borne oceanographic sensors and their potential place within an ocean observing system is reviewed initially. Then, I describe the Series 9000 CTD-SRDL in detail, discussing its performance in the lab and during two field experiences with Southern elephant seals and Weddell seals (Leptonychotes weddellii ). Following this, a detailed study of the ACC frontal system in the South Atlantic is presented that uses merged Argo float data and CTD-SRDL data. The structure of the frontal field revealed by this unique dataset is examined, and unprecedented insight into its variability is obtained. Amongst the important findings is that, contrary to most climate models, our in situ data suggest a northward shift of the ACC east of 40W in 2004 and 2005 compared to previous work. Next, two CTD-SRDL sections are presented to identify the locations of the ACC fronts across Drake Passage, and an empirical relationship between upper ocean temperature and baroclinic mass transport is used to determine the transport through Drake Passage at the times of the sections. This technique is a powerful complement to more conventional means of data collection in this region, especially given the ability of the seals to conduct "sections" at times when ship-based fieldwork is logistically most challenging, i.e. in the winter time. The CTD-SRDLs do not only record hydrographic data, but simultaneously record seal movements and diving behavior. This enables insight to be obtained on the behavioral and physiological responses of Southern elephant seals to spatial environmental variability throughout their circumpolar range. The resulting energetic consequences of these variations could help explain recently observed spatially varying population trends. With a stable population at South Georgia and declining populations at Kerguelen and Macquarie Island. This study also highlights the benefits to the sensorcarrying animals themselves by showing the usefulness of this approach in examining the sensitivity of top predators to global and regional-scale climate variability. More importantly, I conclude that, by implementing animal-borne sensors into ocean observing strategies, we not only gain information about global ocean circulation and enhance our understanding of climate and the corresponding heat and salt transports, but at the same time we increase our knowledge about ocean’s top predators, their life history and their sensitivity to climate change.

2008-11-27T00:00:00Z Boehme, Lars In this thesis, I describe large-scale and small-scale features of the Antarctic Circumpolar Current (ACC) by merging conductivity-temperature-depth (CTD) data obtained by novel animal-borne sensors with data obtained by more conventional means. Twenty-one CTD-Satellite Relay Data Loggers (CTD-SRDLs) were attached to Southern elephant seals (Mirounga leonina) on South Georgia in 2004 and 2005. This was part of a larger international study (Southern Elephant Seals as Oceanographic Samplers; SEaOS), in which I played a major role in developing the oceanographic approach used to integrate physical data from a range of sources, and the means to link biological findings to oceanographic parameters. The development of animal-borne oceanographic sensors and their potential place within an ocean observing system is reviewed initially. Then, I describe the Series 9000 CTD-SRDL in detail, discussing its performance in the lab and during two field experiences with Southern elephant seals and Weddell seals (Leptonychotes weddellii ). Following this, a detailed study of the ACC frontal system in the South Atlantic is presented that uses merged Argo float data and CTD-SRDL data. The structure of the frontal field revealed by this unique dataset is examined, and unprecedented insight into its variability is obtained. Amongst the important findings is that, contrary to most climate models, our in situ data suggest a northward shift of the ACC east of 40W in 2004 and 2005 compared to previous work. Next, two CTD-SRDL sections are presented to identify the locations of the ACC fronts across Drake Passage, and an empirical relationship between upper ocean temperature and baroclinic mass transport is used to determine the transport through Drake Passage at the times of the sections. This technique is a powerful complement to more conventional means of data collection in this region, especially given the ability of the seals to conduct "sections" at times when ship-based fieldwork is logistically most challenging, i.e. in the winter time. The CTD-SRDLs do not only record hydrographic data, but simultaneously record seal movements and diving behavior. This enables insight to be obtained on the behavioral and physiological responses of Southern elephant seals to spatial environmental variability throughout their circumpolar range. The resulting energetic consequences of these variations could help explain recently observed spatially varying population trends. With a stable population at South Georgia and declining populations at Kerguelen and Macquarie Island. This study also highlights the benefits to the sensorcarrying animals themselves by showing the usefulness of this approach in examining the sensitivity of top predators to global and regional-scale climate variability. More importantly, I conclude that, by implementing animal-borne sensors into ocean observing strategies, we not only gain information about global ocean circulation and enhance our understanding of climate and the corresponding heat and salt transports, but at the same time we increase our knowledge about ocean’s top predators, their life history and their sensitivity to climate change. Harris, R.N. http://hdl.handle.net/10023/432 2010-12-06T15:44:07Z 2007-11-30T00:00:00Z

Abstract: Grey seal diet was last comprehensively studied in western Scotland in 1985. Since then, the grey seal population has increased by approximately 30% and relative abundance of fish stocks in the area has changed markedly. The aim of this study was to provide a current assessment of diet to inform policy issues related to the impact of the still increasing grey seal population on commercial fish populations. During 2002, nine sampling trips totalling 56 days were completed around western Scotland, resulting in the collection of 1,589 grey seal scats. Forty-nine prey species were recorded in these samples revealing that grey seals on the west coast of Scotland remain highly catholic in their diet. Seasonal and regional variation in diet composition was assessed and the annual consumption of commercial fish species estimated. Proportions, by weight, of prey species indicated that gadoids were the main prey. Sandeels were also an important component of the diet. Comparisons between 1985 and 2002 revealed many similarities in diet composition but declines in the importance of sandeels, ling and megrim were balanced by increases in haddock, lemon sole, pelagic species and several benthic species. Changes in the size of fish stocks partially explain some of these changes. One exception is cod, which, despite very low abundance in 2002, formed a significant part of grey seal diet in western Scotland. Results from this study highlight the need for better methods for assessing absolute stock abundances for ‘critical’ fish species west of Scotland. Reducing the uncertainty over estimates of grey seal population size would also improve consumption estimates. The results from this study will be important to conservation and fisheries managers in Scotland.

2007-11-30T00:00:00Z Harris, R.N. Grey seal diet was last comprehensively studied in western Scotland in 1985. Since then, the grey seal population has increased by approximately 30% and relative abundance of fish stocks in the area has changed markedly. The aim of this study was to provide a current assessment of diet to inform policy issues related to the impact of the still increasing grey seal population on commercial fish populations. During 2002, nine sampling trips totalling 56 days were completed around western Scotland, resulting in the collection of 1,589 grey seal scats. Forty-nine prey species were recorded in these samples revealing that grey seals on the west coast of Scotland remain highly catholic in their diet. Seasonal and regional variation in diet composition was assessed and the annual consumption of commercial fish species estimated. Proportions, by weight, of prey species indicated that gadoids were the main prey. Sandeels were also an important component of the diet. Comparisons between 1985 and 2002 revealed many similarities in diet composition but declines in the importance of sandeels, ling and megrim were balanced by increases in haddock, lemon sole, pelagic species and several benthic species. Changes in the size of fish stocks partially explain some of these changes. One exception is cod, which, despite very low abundance in 2002, formed a significant part of grey seal diet in western Scotland. Results from this study highlight the need for better methods for assessing absolute stock abundances for ‘critical’ fish species west of Scotland. Reducing the uncertainty over estimates of grey seal population size would also improve consumption estimates. The results from this study will be important to conservation and fisheries managers in Scotland. Cunningham, Louise http://hdl.handle.net/10023/326 2012-10-24T14:21:44Z 2007-06-22T00:00:00Z

Abstract: Managing a wild population effectively requires knowledge of the abundance and behaviour of the species. Harbour seals (Phoca vitulina) are usually counted when they come ashore at haul-out sites, and so it is important to understand how the number of seals counted at this time relates to total population size. Satellite telemetry studies confirmed that harbour seals on the west coast of Scotland showed a degree of site fidelity and coastal foraging. Most trips taken by tagged animals involved travelling only 10-30 km from haul-outs and lasted less than a day (mean 21.07 hours, SE = 0.54), although some seals travelled over 100 km. Eighteen percent of the time these tagged seals spent hauled out was in the Special Area of Conservation where they were caught. Individual seals can be recognised from their unique pelage patterns using computer-assisted photo-identification. Capture histories for adult harbour seals at a site in north-west Scotland indicated that the number of seals using the study area between April and October was 3.4 times higher than the number counted during an aerial survey made during the August moult. In the UK, aerial surveys of harbour seals are usually conducted during the first three weeks of August, when seals are moulting. These counts have a coefficient of variation of around 15%. Land-based counts made at study sites on the north-west coast of Scotland indicated that the number of seals hauled out was most consistent during the moult, but highest counts were from the pupping period. Analysis of moult counts indicated that starting surveys one week earlier (on 7th August) and surveying 1½ hours earlier in the tidal cycle would reduce the count variation. There was spatial, seasonal, diurnal and sex-related variation in the proportion of time harbour seals hauled out. Thus the relationship between counts and total population size is likely to vary spatially and temporally. This variation should be included in the estimates of the CV of correction factors. A 5% annual change in harbour seal population size was predicted to take around 14 years to detect based on annual surveys and a CV = 0.15. This detection period increases when monitoring methods with lower precision are used, or surveys are made less frequently. Trends in seal abundance at pairs of haul-out sites were not synchronous and so it is unlikely that counts from small land-based protected areas, such as Special Areas of Conservation, can be used to monitor overall population status.

2007-06-22T00:00:00Z Cunningham, Louise Managing a wild population effectively requires knowledge of the abundance and behaviour of the species. Harbour seals (Phoca vitulina) are usually counted when they come ashore at haul-out sites, and so it is important to understand how the number of seals counted at this time relates to total population size. Satellite telemetry studies confirmed that harbour seals on the west coast of Scotland showed a degree of site fidelity and coastal foraging. Most trips taken by tagged animals involved travelling only 10-30 km from haul-outs and lasted less than a day (mean 21.07 hours, SE = 0.54), although some seals travelled over 100 km. Eighteen percent of the time these tagged seals spent hauled out was in the Special Area of Conservation where they were caught. Individual seals can be recognised from their unique pelage patterns using computer-assisted photo-identification. Capture histories for adult harbour seals at a site in north-west Scotland indicated that the number of seals using the study area between April and October was 3.4 times higher than the number counted during an aerial survey made during the August moult. In the UK, aerial surveys of harbour seals are usually conducted during the first three weeks of August, when seals are moulting. These counts have a coefficient of variation of around 15%. Land-based counts made at study sites on the north-west coast of Scotland indicated that the number of seals hauled out was most consistent during the moult, but highest counts were from the pupping period. Analysis of moult counts indicated that starting surveys one week earlier (on 7th August) and surveying 1½ hours earlier in the tidal cycle would reduce the count variation. There was spatial, seasonal, diurnal and sex-related variation in the proportion of time harbour seals hauled out. Thus the relationship between counts and total population size is likely to vary spatially and temporally. This variation should be included in the estimates of the CV of correction factors. A 5% annual change in harbour seal population size was predicted to take around 14 years to detect based on annual surveys and a CV = 0.15. This detection period increases when monitoring methods with lower precision are used, or surveys are made less frequently. Trends in seal abundance at pairs of haul-out sites were not synchronous and so it is unlikely that counts from small land-based protected areas, such as Special Areas of Conservation, can be used to monitor overall population status.